小干扰RNA沉默YAP基因对人牙周膜干细胞增殖凋亡的影响
Effects of YAP-small interfering RNA on the proliferation and apoptosis of human periodontal ligament stem cells
作者：唐翠竹,文勇,顾伟亭,张冰,张云鹏,姬雅雯,徐欣,
Author：Tang Cuizhu, Wen Yong, Gu Weiting, Zhang Bing, Zhang Yunpeng, Ji Yawen, Xu Xin,
收稿日期：2015-05-04 年卷（期）页码：2015,33(6):622-622-626
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：小干扰RNA,YAP基因,人牙周膜干细胞,增殖,凋亡,
Key words：small interfering RNA,YAP gene,humanperiodontal ligament stem cells,proli-feration,apoptosis,
基金项目：国家自然科学基金资助项目（81300885）；山东省自然科学基金资助项目（ZR2013HQ052，ZR2013HM086）；山东省医药卫生发展计划基金资助项目（2013WS0212）；山东大学青年学者未来计划基金资助项目（2015WLJH53）；山东大学基本科研业务费专项基金资助项目（2015QY003）
中文摘要
目的 本研究通过小干扰RNA（siRNA）沉默人牙周膜干细胞（hPDLSCs）YAP基因，观察YAP基因沉默后对细胞增殖凋亡的影响。方法 将化学合成siRNA序列以脂质体LipofectamineTM 2000介导瞬时转染hPDLSCs，采用实时荧光定量逆转录聚合酶链反应（RT-PCR）、Western blot检测转染后YAP表达水平，细胞增殖活性检测试剂盒（CCK-8）检测siRNA在体外对hPDLSCs增殖能力的影响，流式细胞仪检测细胞周期及凋亡率的变化。采用SPSS 19.0软件对数据进行处理，设PPPP
英文摘要
Objective To investigate the effects of small interfering RNA (siRNA) targeting YAP on the proliferationand apoptosis of human periodontal ligament stem cells (hPDLSCs). Methods Synthesized sequences of siRNA were transfectedinto hPDLSCs by LipofectamineTM 2000. The expression of YAP was identified by using real-time quantitative reversetranscription-polymerase chain reaction (RT-PCR) and Western blot analysis. Proliferation activity was detected by usingcell counting kit-8 (CCK-8). Changes in the cell cycle and apoptosis rate were detected by using flow cytometry. Resultswere analyzed by using SPSS 19.0, andPPPP
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