特定序列寡核苷酸MT01对牙龈卟啉单胞菌感染的成骨细胞的增殖、细胞周期及凋亡的影响
Effect of specific sequence oligodeoxynucleotide MT01 on the proliferation, apoptosis, and cell cycle of osteoblasts invaded by Porphyromonas gingivalis
作者：于海蛟,申玉芹,刘引,高涵,周岳,胡天琦,林崇韬
Author：Yu Haijiao, Shen Yuqin, Liu Yin, Gao Han, Zhou Yue, Hu Tianqi, Lin Chongtao
收稿日期：2015-05-26 年卷（期）页码：2015,33(6):617-617-621
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：特定序列寡核苷酸MT01,牙龈卟啉单胞菌,成骨细胞,细胞周期,凋亡,
Key words：specific sequence oligodeoxynucleotide MT01,Porphyromonas gingivalis,osteoblast,cell cycle,apoptosis,
基金项目：国家自然科学基金面上项目资助项目（81371153）；吉林省科技厅自然科学基金面上项目资助项目（20150101173JC）
中文摘要
目的 通过观察MT01对感染状态下成骨细胞细胞增殖、细胞周期及凋亡的影响，探讨MT01对牙周致病菌感染的成骨细胞生物学性能的影响。方法 以人成骨细胞MG63为目的细胞，分别与PBS、MT01、CpG ODN、甲硝唑和庆大霉素共培养3 h后，按感染复数100∶1的比例加入牙周致病菌牙龈卟啉单胞菌共培养，检测培养24 h和48 h后MG63细胞的增殖、细胞周期及凋亡，以PBS作为空白对照，以牙龈卟啉单胞菌和PBS共培养作为阴性对照。结果MT01+牙龈卟啉单胞菌组细胞增殖高于空白对照组（P1期细胞百分比低于阴性对照组，而S期细胞百分比高于阴性对照组（PP1期细胞百分比，促使细胞进入S期并抑制细胞早期凋亡。
英文摘要
Objective This aimed to investigate the effect of specific sequence oligodeoxynucleotide MT01 on the biological properties of osteoblasts invaded byPorphyromonas gingivalis(P. gingivalis) by evaluating proliferation, cell cycle, and apoptosis. Methods MG63 osteoblasts were recovered and incubated with MT01, CpG ODN, metronidazole (MNZ), and gentamicin (GEN) for 3 h.P. gingivalis(the multiplicity of infection was 100∶1) was added subsequently and cocultured for another 24 and 48 h. Cells with PBS comprised the blank group, whereas cells withP. gingivaliscomprised the negative controls. Six experimental groups were established: PBS group,P. gingivalisgroup, MT01+P. gingivalisgroup, CpG ODN+P. gingivalisgroup, MNZ+P. gingivalisgroup, and GEN+P. gingivalisgroup. The proliferative ability was measured by methyl thiazolyl tetrazolium assay, and the percentages of apoptosis and cell cycle were examined by flow cytometry. Results Compared with the blank group, proliferation increased significantly in the MT01+P. gingivalisgroup (P1phase and higher at the S phase in the MT01+P. gingivalisgroup compared with the results in theP. gingivalisgroup (PP. gingivalisgroup was significantly lower than that in theP. gingivalisgroup (P1phase, increase the ratio of the S phase, and inhibit the early apoptosis of osteoblasts invaded byP. gingivalis.
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