小型猪下颌骨放射性骨坏死动物模型的建立
Establishment of a miniature pig model of mandibular osteoradionecrosis
作者：李松,房殿吉,王占义,翟璐璐,宁兆荣,郭延伟
Author：Li Song, Fang Dianji, Wang Zhanyi, Zhai Lulu, Ning Zhaorong, Guo Yanwei
收稿日期：2015-04-10 年卷（期）页码：2015,33(6):570-570-574
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：放射性骨坏死,下颌骨,小型猪,动物模型,
Key words：osteoradionecrosis,mandible,miniature pig,animal model,
基金项目：国家自然科学基金资助项目（81170935）
中文摘要
目的 探讨利用小型猪建立下颌骨放射性骨坏死（ORN）动物模型的可行性。方法 应用三维适形放疗（3D-CRT）技术，采用电子直线加速器照射源对24只小型猪右侧下颌骨进行25 Gy和28 Gy一次性照射，于照射结束后2个月拔除双侧下颌第一磨牙，通过定期局部观察、X线、CT检查和组织病理学方法诊断下颌骨ORN的发生。结果 照射结束后3~4个月，拔牙后的小型猪先后全部发生了下颌骨ORN，28 Gy+拔牙组症状较重。结论 25 Gy单一剂量放射小型猪下颌骨+拔牙能建立有效的、可信的下颌骨ORN模型，可用于ORN病因学及治疗等方面的进一步研究。
英文摘要
Objective To establish a miniature pig model of mandibular osteoradionecrosis (ORN). Methods A single dose of irradiation (IR25 Gy, 28 Gy) was delivered via an electronic linear accelerator to the right mandible of 24 miniature pigs by utilizing three dimensional conformal radiotherapy (3D-CRT). The first molar of the mandible was extracted 2 months after radiation. Mandibular ORN was diagnosed through gross observation, X-ray film, CT, and histopathologic examination. Results At 3–4 months after radiation, all animals suffered from mandibular ORN after dental extraction. The symptoms of ORN were more severe in the 28 Gy group than in the 25 Gy group. Conclusion A miniature pig model of mandibular ORN can be established through 25 Gy irradiation+dental extraction.
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