正畸应力作用下牙龈肌成纤维细胞表达的初步研究
Expression of myofibroblast in gingival after orthodontic loading
作者：孟耀,刘曼,邓倩楠
Author：Meng Yao,Liu Man,Deng Qiannan
收稿日期：2019-03-15 年卷（期）页码：2019,37(5):537-537-540
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：正畸,牙龈,肌成纤维细胞,α-平滑肌肌动蛋白,
Key words：orthodontics,gingival,myofibroblast,α-smooth muscle actin,
基金项目：深圳市科技创新委员会基础研究项目(JCYJ201802281-64957104)
中文摘要
目的 初步探讨正畸应力加载后牙龈肌成纤维细胞的表达情况。方法 选取8例正畸应力加载后需要拔除牙牙龈组织,以应力加载前拔除牙牙龈组织为对照,进行α-平滑肌肌动蛋白（α-SMA）、Ⅰ型胶原、Ⅲ型胶原免疫组织化学染色。然后进行测量和统计分析。结果 正畸应力加载前牙龈组织内Ⅰ型胶原、Ⅲ型胶原免疫组化染色阳性,α-SMA除血管上皮外,其余为阴性表达。正畸应力加载后,Ⅰ型胶原、Ⅲ型胶原表达明显上升,差异有统计学意义（PP结论 正畸应力加载后,牙龈组织内肌成纤维细胞出现表达,其可能在正畸牙术后复发中发挥一定作用。
英文摘要
ObjectiveTo examine the expression of myofibroblast in gingival after orthodontic loading.MethodsEight patients were selected as experimental group and treated with orthodontic force for 4 months. Ten patients were selected as the control group, were not loaded. The gingival protein expressions of collagen typeⅠ, collagen type Ⅲ, α-smooth muscle actin (α-SMA) were evaluated by immunohistochemistry method.ResultsPositive expressions of collagen typeⅠ, collagen type Ⅲ were founded, while no positive staining for α-SMA in the gingival tissue except vascular epithelium before loading. In experimental group, collagen type I and collagen type Ⅲ were increased after orthodontic loading (PPConclusionThe myofibroblast exists in gingival tissue after orthodontic loading, and it may be concerned with orthodontic teeth relapse.
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