microRNA-125b在舌鳞状细胞癌中的表达及意义
Expression and significance of microRNA-125b in tongue squamous cell carcinoma
作者：王健,闫广鹏,郭超,李军
Author：Wang Jian,Yan Guangpeng,Guo Chao,Li Jun
收稿日期：2019-04-25 年卷（期）页码：2020,38(1):11-11-16
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：microRNA-125b,舌鳞状细胞癌,实时荧光定量聚合酶链反应,原位杂交,
Key words：microRNA-125b,tongue squamous cell carcinoma,real time fluorescence-quantitative polymerase chain reaction,in situ hybridization,
基金项目：新疆维吾尔自治区自然科学基金(2016D01C099)
中文摘要
目的 检测舌鳞状细胞癌（TSCC）中microRNA-125b的表达,分析与TSCC患者临床病理特征的关系。方法 采用实时荧光定量聚合酶链反应（RT-qPCR）检测35例TSCC组织及对应的癌旁正常组织中microRNA-125b的表达情况,并分析microRNA-125b在TSCC组织中的表达与临床特征的关系。采用原位杂交技术（ISH）检测35例TSCC组织及对应的癌旁正常组织切片中microRNA-125b基因的表达水平。结果 RT-qPCR结果显示,TSCC组织和癌旁正常组织中microRNA-125b的相对表达量分别为2.32±0.69和0.87±0.32,TSCC组织中microRNA-125b的表达显著高于癌旁正常组织,差异有统计学意义（PPP结论 microRNA-125b在35例TSCC患者中高表达,并与临床分期相关,提示microRNA-125b表达上调可能参与TSCC的发生发展。
英文摘要
ObjectiveThe expression of microRNA-125b in tongue squamous cell carcinoma (TSCC) was detected and analyzed for its relationship with the clinicopathological features of TSCC.MethodsReal time fluorescence-quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of microRNA-125b in 35 TSCC tissues and adjacent normal tissues from 35 TSCC cases. The relationship between the expression of microRNA-125b in TSCC tissues and the clinicopathological features of patients with TSCC was analyzed. In situ hybridization (ISH) was used to detect the expression level of microRNA-125b gene in the TSCC tissues and adjacent normal tissues.ResultsRT-qPCR results showed that the relative expression levels of microRNA-125b in the TSCC issues was 2.32±0.69, and that of normal tissues was 0.87±0.32. The statistical results showed that the expression level of microRNA-125b was significantly higher in the TSCC tissues than in the normal tissues (PPConclusionMicroRNA-125b is highly expressed in TSCC and associated with TNM stage, suggesting that high microRNA-125b expression may be involved in the development of TSCC.
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