Gli1阳性细胞在牙周组织发育中的时空分布特点及功能研究
Temporal and spatial distribution of Gli1+ cells and their function during periodontal development
作者：王韵, 谢旭东, 许春梅, 王骏
Author：Wang Yun, Xie Xudong, Xu Chunmei, Wang Jun
收稿日期：2019-10-15 年卷（期）页码：2020,38(2):128-128-132
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：Gli1,牙周组织,发育,谱系示踪,干细胞,
Key words：Gli1,periodontal tissue,development,lineage tracing,stem cell,
基金项目：国家自然科学基金(81700980);四川省科技计划项目(2019YJ0097);中国博士后科学基金(2017M623048);四川大学专职博士后研发基金(2018SCU12018)
中文摘要
目的 利用转基因小鼠探究牙周膜内Gli 阳性（Gli1 +）细胞的表达分布以及Gli1 +细胞在牙周组织发育过程中的作用。方法 收集3、6和8周龄Gli1 lacZ/+小鼠下颌骨,通过β-半乳糖苷酶组织化学染色（X-gal染色）观察牙周膜内Gli1 +细胞的时间和空间分布特点。然后,通过注射他莫昔芬诱导3周龄Gli1-Cre ERT2/+;R26R tdTomato/+小鼠表达红色荧光蛋白tdTomato,对Gli1 +细胞及其子代细胞（tdTomato +细胞）进行动态追踪。结果 3周龄小鼠牙周膜内分布大量Gli1 +细胞,随着年龄的增长,Gli1在牙周膜内的数量逐渐减少（P<0.05）。tdTomato +细胞随着诱导时间的延长,数量逐渐增加（P<0.05）,且逐渐分化成熟为成纤维细胞、牙骨质细胞和骨细胞。结论 牙周膜内Gli1 +细胞具有多向分化的潜能,是牙周组织发育过程中重要的细胞来源。
英文摘要
ObjectiveThis study aimed to investigate the distribution of Gli1+cells in the periodontal ligament (PDL) and to evaluate their contribution in the development of periodontal tissue by using transgenic mouse lines.MethodsGli1lacZ/+mice were harvested at different ages (3, 6, and 8 weeks), and the temporal and spatial distribution patterns of Gli1+PDL cells were revealed by X-gal staining. Afterward, 3-week-old Gli1-CreERT2/+;R26RtdTomato/+mice were administered with tamoxifen, and the fates of Gli1+cells and their descendants were traced during periodontal development.ResultsA large number of Gli1+cells were detected in the PDL of the 3-week-old mice; however, their number significantly decreased from 3 weeks to 8 weeks (P<0 .05). cell lineage tracing data showed that the descendants of gli1+cells dramatically increased from 3 weeks to 8 weeks (P<0 .05) and gradually differentiated into fibroblasts, cementocytes, and osteocytes.ConclusionThe multi-differentiation potential of Gli1+PDL cells was revealed, indicating that Gli1+cells are an important cell source for periodontal development.
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