ObjectiveTo investigate the effect of oral microscope-assisted surface decontamination on implantsin vitro.MethodsTwelve implants that fell off because of severe peri-implantitis were collected, and decontamination was carried out on the surfaces of implants through curetting, ultrasound, titanium brushing, and sandblasting at 1×, 8×, or 12.8× magnifications. The number and sizes of residues on the implants’ surfaces after decontamination were determined, and the decontamination effect was analyzed according to the thread spacing in the different parts of the thread.Results1) The 8× and 12.8× groups scored lower for implant surface residues than the 1× group (P<0 .000 1), and the 12.8× group scored lower than the 8× group (P<0 .001); 2) no difference in residue score was found between the wide and narrow thread pitch (P>0.05), and the 8× and 12.8× groups had lower scores than the 1× group (P<0 .001); 3) the lowest number of contaminants was observed at the tip of the thread, whereas the highest was observed below the thread, and the difference was significant (P<0 .001). however, the thread pitch had no effect on the number of contaminants in different areas (P>0.05); 4) the residue scores of the 8× and 12.8× groups were lower than those of the 1× group at the thread tip and above, sag, and below the thread of the implants (P<0 .05).ConclusionResidues on the surfaces of contaminated implants can be effectively removed by using an oral microscope. After decontamination, the residues of pollutants were mainly concentrated below the thread of the implants, and the thread pitch of the implants had no significant effect on the residues.