ObjectiveIn this study, TeeTester digital occlusal analysis system was used to analyse the occlusal characteristics of chewing side preference (CSP) and non-CSP patients with TMD symptoms.MethodsAccording to the questionnaire and oral examination, 80 subjects were screened and divided into four groups: 20 CSP patients with TMD symptoms, 20 CSP patients without TMD symptoms, 20 non-CSP patients with TMD symptoms, 20 non-CSP patients without TMD symptoms. TeeTester digital occlusal system was applied to measure the occlusal characteristics in all four groups, including total contect area (TCA), total occlution force (TOF), distribution of occlusal force (DOF), asymmetric index of occlusal force (AOF), occlusal time (OT), disclusion time (DT) and center of occlusal force(COF). SPSS 25.0 was used to analyze the statistical data.ResultsAt intercuspal position, the data reveals significant differences in the maximum TOF and TCA among the four groups (P<0 .05). there was significant difference in the distribution of occlusal force between the preferred side and the other side of csp patients with or without tmd symptoms (P<0 .05). among the four groups, there were statistically significant difference in the position of the center point of occlusal force, the direction of the center point of occlusal force, and the vertical distance between the center point of occlusal force and the center line (P<0 .05). the cof, maximum tof and tca of the preferred side of dentition were significantly positive correla⁃ted with csp (P<0 .05), according to spearman correlation analysis. however, the maximum tof and tca of the both side of dentition were significantly negative correlated with tmd symptoms (P<0 .05).Conclusion1) The occlusal characteristics of CSP patients and non-CSP patients with TMD symptoms are different. TeeTester digital occlusal analysis system provides objective parameters for the dynamic evaluation of occlusion. 2) Among all the occlusal cha⁃racteristics, maximum TOF, TCA and COF of the preferred side of dentition were positively correlated with CSP. The abnormal occlusal characteristics (maximum TOF and TCA of the whole dentition) caused by CSP are related to TMD symptoms.