Most of the traditional radio models are invalid due to the special propagation environment on lunar.A novel path loss prediction method for lunar surface was proposed. Firstly,a theoretical segment model according to communication distance was established and three propagation scenarios were analyzed. Then the multiple isolated obstacles on propagating path were considered, which would not only lead to an additional transmission loss but also affect the segment parameters. The present method was applied on a real lunar surface scenario provided by National Aeronautics and Space Administration (NASA). The simulation results showed that the prediction results are very close to measurements by Apollo project. This segment prediction model could be very useful on system parameters design and performance analysis of lunar communication system.