In order to deal with the diversity and unnormalization of the preparation of modified asphalt samples in the fluorescence microscopy observation, the influence factors on the observation results were analyzed through test in various kinds of preparation methods, such as microscopic glass, heated or unheated microslide and so on. Ensuring that samples won’t be beared by the external disturbances such as force and temperature is the necessary condition of acquiring real morphostructure. The flatness of sample’s surface is the precondition and foundation of collecting microscopic images effectively. Rationing and figuration are effective ways to guarantee the good reproducibility morphostructure of modified asphalt. Finally, samples’ preparation requirements that may guarantee the microstructure of modified asphalt better reproducibility and better stability were put forward.
