A type of bioreactor using wood sawdust as solid support inoculated with Thiobacillus sp. was developed for the removal of H2S in waste gases. The bacteria were isolated from a hot spring in Sichuan province. The results showed that wood sawdust provided high bacteria adsorption capacity. The maximum removal capacity was 530 g·m-3·h-1 (removal efficiency of 80 %) for gas flow rates in the low rage of 6~18 L·h-1. However, in the high gas flow rates of 21~36 L·h-1, there was no evident critical point of inlet S-loading. The effect on the production of SO42- was also studied. The concentration of SO42- was increased as gas flow rate increasing at the constant inlet S-loading. And when the gas flow rate was constant, the concentration of SO42- was high at low inlet S-loading (0.95) between experimental data and the predicted curves were found.