The autostereoscopic display is an important display technology method of sub-pixel arrangement for the multi-view autostereoscopic display based on step parallax barriers was proposed. The view number matrixes were built and the sub-pixel arrangement results were obtained for different inclined angles of parallax barriers and different view numbers of images.The rules of sub pixel arrangements were analyzed. A demo of a multi-view autostereoscopic display based on step parallax barriers using the sub-pixel arrangements presented very good stereo images.