College of Life Sciences, Sichuan University, Chengdu 610065, China) In this experiment, it was designed to investigate the effects of overexpression of OsPGIP gene on the Brassica napus growth and development. The seed quality index and seeding growth condition were determined in this study. We also observed the flower organ morphology by Nikon SMZ18 fluorescence microscope and detected OsPGIP gene expression level by RT-PCR. Besides, this experiment also includes morphological observation of flower organs, determination activities of antioxidant enzymes such as POD, SOD, CAT, etc., and statistics of important agronomic characters such as the number of pods, effective branches and days of first flowering. The results showed that there were no obvious defects in seed quality and seedling growth of T1 generation transgenic families, the development of flower organs was normal and the days of first flowering were shortened about 8 days compared with the wild type. Our data indicate that overexpression of PGIP gene could significantly shorten the first flowering days of rape, but had no significant effect on its normal growth and seed setting.
