高效液相色谱法测定聚氨酯合成革中N,N-二甲基甲酰胺
Determination of N,N-Dimethylformamide in Polyurethane Synthetic Leather by High Performance Liquid Chromatography
作者：胡苹, 曾建伟, 李大刚, 陈崇城, 刘丽丽, 王开明, 詹迎旭
Author：HU Ping, ZENG Jianwei, LI Dagang, CHEN Chongcheng, LIU Lili, WANG Kaiming, ZHAN Yingxu
收稿日期：2019-10-18 年卷（期）页码：2020,30(2):64-67
期刊名称：皮革科学与工程
Journal Name：Leather Science and Engineering
关键字：高效液相色谱法,聚氨酯合成革,N,N-二甲基甲酰胺,
Key words：HPLC,polyurethane synthetic leather,N,N-Dimethylformamide,
基金项目：
中文摘要
建立了高效液相色谱法（HPLC）快速测定聚氨酯合成革中N,N-二甲基甲酰胺的方法。样品经纯水超声提取,滤膜过滤后直接进样,高效液相色谱法定性定量分析。优化了仪器条件,确定选择甲醇和水为流动相（20%∶80%）,检测波长为205 nm。结果表明,N,N-二甲基甲酰胺在0.1~10 mg/L浓度范围内呈良好的线性,相关系数（r2）为0.9999,方法检出限为0.23 mg/kg,方法定量限为0.78 mg/kg,在3个加标水平下的平均回收率为96.2%~99.8%,相对标准偏差（RSD,n=6）为2.1%~5.1%,该方法简单,快速,准确,稳定可靠,适用于聚氨酯合成革中N,N-二甲基甲酰胺的检测。
英文摘要
A method for the determination of N,N-Dimethylformamide in polyurethane synthetic leather was developed by high performance liquid chromatography. Sample was ultrasonically extracted with pure water. After being purified with membrane filter, the extract was quantitatively determined by HPLC method. Instrument conditions were optimized. The optimized mobile phases were methanol and water (20%∶80%), and the detector at 205 nm was used. The calibration curves of N,N-Dimethylformamide was linear in the range of 0.1-10 mg/L, and correlation coefficients (r2) was 0.9999. The detection limit was 0.23 mg/kg, and the limit of quantitation was 0.78 mg/kg. The spiked average recoveries varied from 96.2% to 99.8% at three spiked levels with relative standard deviation (RSD,n=6) of 2.1%-5.1%. The proposed method was simple, rapid, accurate and stable, reproducible for determination of N,N-Dimethylformamide in polyurethane synthetic leather.
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