p38MAPK-siRNA脂质体递药系统的制备及体外抗炎作用的初步研究
Preparation and in vitro anti-inflammatory effect study of p38MAPK-siRNA liposome drug delivery system
作者：王元芳;汪家鼎;吴青思;李林;张志荣;
Author：
收稿日期： 年卷（期）页码：2020,35(02):-131-134
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：p38MAPK-siRNA;阳离子脂质体;超高速离心法;探头超声;包封率;抗炎;RAW264.7巨噬细胞
Key words：
基金项目：国家自然科学基金资助项目(批准号:81690261)
中文摘要
目的制备一种递送p38MAPK-siRNA的阳离子脂质体,研究其对脂多糖(LPS)诱导的小鼠RAW264.7炎症细胞模型的抗炎作用。方法采用薄膜分散-超声法制备p38MAPK-siRNA阳离子脂质体;用超速离心法分离制剂和游离p38MAPK-siRNA,通过荧光标记法检测脂质体对p38MAPK-siRNA的包封率;选用小鼠RAW264.7单核巨噬细胞为细胞模型,考察制剂的体外抗炎作用。结果成功制备了阳离子脂质体,p38MAPK-siRNA与其的包封率为87.29%;制剂具有一定的体外抗炎作用,通过抑制p38MAPK信号通路的活化,降低相关炎症因子IL-6、TNF-α的表达水平,从而发挥细胞抗炎作用。结论以小分子干扰RNA作为治疗手段的新型递药系统在未来药物治疗领域具有巨大的潜力。
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