雷公藤红素与紫杉醇白蛋白纳米粒的制剂研究
Preparation study of celastrol and paclitaxel co-loaded albumin nanoparticles
作者：肖林玉;薛圆;罗诗;杜正午;李佩纹;张凌;王力;田伯乐;张志荣;
Author：
收稿日期： 年卷（期）页码：2020,35(02):-135-139
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：雷公藤红素;紫杉醇;白蛋白纳米粒;胰腺癌;共载;处方筛选;细胞摄取;长循环
Key words：
基金项目：国家自然科学基金资助项目(批准号:81690261)
中文摘要
目的制备雷公藤红素(CLT)与紫杉醇(PTX)共载的长循环白蛋白纳米粒,并对其进行表征和初步小鼠胰腺癌细胞的摄取研究。方法采用单因素筛选法依次筛选有机溶剂种类、白蛋白用量、DSPE-PEG用量、CLT与PTX的比例、大豆油和e80磷脂辅料的用量以及制备工艺,并对纳米粒粒径、电位、形态、包封率和载药量进行表征。同时应用流式细胞术对其细胞摄取情况进行研究。结果筛选出最优处方,成功制备了均一稳定的CLT与PTX共载长循环白蛋白纳米粒(CP-AN),其粒径约128 nm,PDI小于0.2,Zeta电位约-20 mV,对CLT的包封率约85%、PTX的包封率约75%。DSPE-PEG在一定程度上降低了细胞对纳米粒的摄取效率。结论 CLT与PTX具有良好的相容性,能应用简单的处方和超声工艺制成共载长循环白蛋白纳米粒。
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