ObjectiveTo evaluate the coagulation factors and thrombelastography (TEG) of leukocytes reduced apheresis platelets during the final storage period, in order to guide the reasonable clinical transfusion of leukocytes reduced apheresis platelets.
MethodsFrom June 2017 to June 2019, a total of 65 leukocytes reduced apheresis platelets collected from Rizhao Blood Center were selected into this study by random sampling method. The leukocytes reduced platelets were collected by MCS+ blood cell separator and its matching pipeline consumables. The platelet count, FⅧ activity, FⅨ activity, and TEG of leukocytes reduced apheresis platelets were tested before storage, at (22±2)℃ shaking storage on the 5th and 7th day. The overall comparison of above indicators at 3 different time points used one-way repeated measurement analysis of variance; and the pairwise comparison of different time points used the least significant difference (LSD) method. The procedures followed in this study were in accordance with the requirements of theWorld Medical Association Declaration of Helsinkirevised in 2013.
ResultsIn this study, before storage, on the 5th and 7th day of storage, the platelet count of 65 leukocytes reduced apheresis platelets were (10.8±0.5)×1011/L, (8.7±1.1)×1011/L, (8.2±1.5)×1011/L, FⅧ activity were (105.9±38.2)%, (57.2±22.4)%, (30.5±17.5)%, FⅨ activity were (97.8±27.5)%, (54.9±19.7)%, (38.4±12.4)%, respectively. Before storage, on the 5th and 7th day of storage, the above 3 indicators were compared, and the overall differences were statistically significant (F=93.90,P<0 .001;F=126.16,P<0 .001;F=141.04,P<0 .001). the differences of above 3 indicators between the 5th day of storage and before storage (lsd-t=10.52,P<0 .001; lsd-t=10.13,P<0 .001; lsd-t=11.76,P<0 .001), all and between the 7th day and 5th day of storage (lsd-t=2.34,P=0.020; LSD-t=5.53,P<0 .001; lsd-t=4.50,P<0 .001), all were statistically significant. ② before storage, on the 5th and 7th day of storage, the teg parameters r value, k value, α angle, and ma value of the 65 leukocytes reduced apheresis platelets were (8.8±0.5)min, (14.1±0.7)min , (16.1±0.6)min; (1.7±0.1)min, (1.8±0.5)min, (1.9±0.6)min; (69.7±3.4)°, (69.1±1.8)°, (69.2±2.6)°, and (86.7±2.6)mm, (82.2±3.1)mm, (80.5±3.3)mm, respectively. the r value, k value, and ma value were compared respectively, and the overall differences were statistically significant (F=2 522.59,P<0 .001;F=3.15,P=0.045;F=73.42,P<0 .001). compared with before storage, the r value were incteased significantly and ma value were reduced significantly on the 5th day of storage, and the differences were statistically significant (lsd-t=49.90,P<0 .001; lsd-t=8.51,P<0 .001). compared with the 5th day of storage, the r value were incteased eignificantly and ma value decreased significantly on the 7th day of storage, and the differences were statistically significant (lsd-t=18.83,P<0 .001; lsd-t=3.22,P=0.002).
ConclusionsDuring the final storage period of leukocytes reduced apheresis platelets, as (22±2)℃ shaking storage on the 5th day, coagulation active components are significantly depleted and coagulation function decreased. Clinical transfusion of leukocytes reduced apheresis platelets in different storage periods should be selected according to different therapeutic purposes.
