破骨细胞相关因子在骨再建过程中表达的定量分析
Quantitative Study of Osteoclastic Related Factors in the Process of Bone Reconstruction
作者：常新;侯志明;柴田恭明;塚崎智雄;山口朗
Author：CHANG Xin1, HOU Zhi-ming2, SIBATA Yasuaki3，TSUKAZAKI Tomoo3，YAMAGUCHI Akira3
收稿日期：2006-04-25 年卷（期）页码：2006,24(02):164-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：肿瘤坏死因子,核因子-κB受体激动剂,抗酒石酸酸性磷酸酶,实时定量聚合酶链反应,骨再建,
Key words：tumornecrosisfactor,receptoractivatorofNF-κBligand,tartrate-resistantacidphosphatase,realtimepolymerasechainreaction,boneremodeling,
基金项目：
辽宁省自然科学基金资助项目（619022）；辽宁省博士启动基金资助项目（2001102069）；日本文部科学省博士后基金资助项
目（02262）
中文摘要
目的 研究肿瘤坏死因子-α（TNF-α）、核因子-?资B受体激动剂（RANKL）、抗酒石酸酸性磷酸酶（TRAP）在骨再建过程中的表达。方法 用8周龄雄性C57BL/6J小鼠建立骨折模型，用RT-PCR法对骨形成过程中TNF-α、RANKL和TRAP的表达进行时程分析。结果 骨折后TNF-α、RANKL、TRAP的表达均上调, 但峰值在不同的时间点出现。TNF-α在骨折后2 d达峰值, RANKL的峰值出现在骨折5 d后, 而TRAP的表达在骨折后10 d达到峰值。结论 骨的再建也有破骨细胞相关因子的参与, 它有赖于成骨和破骨的精确平衡。
英文摘要
Objective To detect the expression of osteoclast related factors, tumor necrosis factor-α（TNF-α）, receptor activator of NF-?资B ligand（RANKL） and tartrate-resistant acid phosphatase（TRAP）, in the process of bone remodeling. Methods 8-week-old male C57BL/6J mice were employed in this study to detect the expression of osteoclast related factors by real time PCR. Results TNF-α, RANKL and TRAP were up regulated in the process of bone remodeling, they reached the peak on day 2，5 and 10 individually after injury. Conclusion Osteoclast related factors also participate in bone remodeling, which depends on the delicate balance between bone formation and bone resorption.
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