HSV-tk与p53基因对涎腺多形性腺瘤细胞的杀伤作用
The Effects of HSV-tk Suicide Gene and Wild-type p53 Gene on Pleomorphic Adenoma Cells of Salivary Gland
作者：王旭,王洁,董福生,董玉英,侯亚丽
Author：WANG Xu1,WANG Jie1,DONG Fu-sheng2,DONG Yu-ying2,HOU Ya-li1
收稿日期：2005-02-25 年卷（期）页码：2005,23(01):65-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：腺瘤,涎腺,基因治疗,野生型p53,单纯疱疹病毒胸苷激酶基因,
Key words：adenoma,salivary gland,gene therapy,wild type p53,herpes simplex virus thymidine kinase gene,
基金项目：
国家自然科学基金资助项目(30271422);国家留学人员科技活动择优资助项目(2002年);河北省自然科学基金资助项目 (C2004000624)
中文摘要
目的 观察腺病毒介导的单纯疱疹病毒胸苷激酶(HSV-tk)基因、野生型p53基因(wt-p53)联合杀伤人涎腺
多形性腺瘤细胞的效果。方法 采用腺病毒介导的HSV-tk基因、wt-p53基因联合转染人涎腺多形性腺瘤细胞,逆
转录聚合酶链式反应(RT-PCR)检测转染后的基因表达;四唑盐比色(MTT)法测定tk与p53基因治疗后对肿瘤细
胞的杀伤作用及旁观者效应,并采用相差显微镜观察基因治疗后肿瘤细胞的形态学变化。结果 单独转染wt-p53
及HSV-tk/GCV基因5 d后,涎腺多形性腺瘤细胞的存活率分别为54%和38%;两者联合转染5 d后,细胞的存活率
降至20%,两者差异有显著性(P
英文摘要
Objective To studythe therapeutic effects of combined gene therapy ofwild type p53 (wt-p53) and herpes simplex virus thymidine kinase (HSV-tk) gene on pleomorphic adenoma cells of salivary gland.Methods Wild type p53 and HSV-tk gene were transfected into human pleomorphic adenoma cells of salivary gland by using recombinant adenovirus vector. The effi- ciency of transfection was checked and gene was expressed by RT-PCR methods. The cell inhibition after transfected was verified by light microscope and MTT.Results The proliferation of the pleomorphic adenoma cells transfected wt-p53 and HSV-tk gene was inhibited and the cell survival rate decreased to 54% and 38% respectively in 5 days. However, whenwt-p53 gene combined withHSV-tk/GCV system, the killing effects was significantly stronger (P
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