人乳牙牙根吸收过程中根面破骨细胞的观察
Observation of Osteoclasts on the Root Surface during Human Deciduous Teeth Resorption
作者：包向军,梁星,陈明,王航,谢志刚,杨晓喻
Author：BAO Xiang-jun1,LIANG Xing2,CHENMing2,WANGHang2,XIE Zhi-gang2,YANGXiao-yu2
收稿日期：2004-08-25 年卷（期）页码：2004,22(04):328-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：乳牙,破骨细胞,形态学,
Key words：deciduous teeth,osteoclasts,morphology,
基金项目：
国家自然科学基金资助项目(30271428);教育部“优秀青年教师资助计划”项目(教人司[2002]40号);高等学校博士学科点专项科研基金资助项目(B1200058)
中文摘要
目的 观察人乳牙根吸收面的破骨细胞形态特点。方法 收集和固定人滞留乳牙,制备硬组织切片,经苏木精-伊红、抗酒石酸酸性磷酸酶染色,在光镜和扫描电镜下观察破骨细胞。结果 在人乳牙根吸收面有多个破骨细胞,呈重叠状态,细胞形态不规则,体积大,细胞周缘可见丝状突起,多核,被吸收的牙本质表面高低不平。结论 人滞留乳牙牙根吸收面存在大量的破骨细胞,是研究人破骨细胞的一个来源。
英文摘要
Objective To observe osteoclasts on the resorbing surface of human deciduous teeth.Methods After fixing the collected deciduous teeth, we prepared the tooth sliceswithout decalcification, treated themwithHE andTRAP dyestuff, and ob- served the osteoclasts under light and scanning electronmicroscope.Results Therewere large quantity of various forms of overlap- ping and huge osteoclastswith many nuclei and silk-like protuberances on the resorbing surface of deciduous teeth. The multinu- cleated osteoclasts align on the surface of coarse dentin.Conclusion On the resorbing surface of human deciduous teeth there are large amount of osteoclasts which can be used as a source of studying human osteoclast.
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