人乳牙破骨细胞降钙素受体mRNA的表达
Investigation of the Expression of Calcitonin Receptor mRNA in Human Osteoclasts on Deciduous Teeth
作者：陈 明1,梁 星2,包向军2,王 航2,孙惠强2,陆邵恒2
Author：CHENMing1, LIANGXing2, BAOXiang-jun2, WANGHang2, SUNHui-qiang2,LUShao-heng2
收稿日期：2004-06-25 年卷（期）页码：2004,22(03):235-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：乳牙,破骨细胞,原位杂交,
Key words：deciduous teeth,osteoclasts,in situ hybridization,
基金项目：
国家自然科学基金资助项目(30271428);教育部“优秀青年教师资助计划”项目(教人司[2002]40号);高等学校博士学科点专项科研基金资助项目(B1200058)
中文摘要
目的 检测人乳牙吸收过程中乳牙根部破骨细胞降钙素受体mRNA的表达。方法 收集和固定人滞留乳牙,用甲苯胺蓝和抗酒石酸酸性磷酸酶(TRAP)染色观察和定位破骨细胞,用原位杂交检测降钙素受体mRNA表达情况。结果 在人乳牙吸收面有多个破骨细胞,破骨细胞TRAP染色阳性并且表达钙素受体mRNA。结论 人乳牙吸收面破骨细胞可以表达降钙素受体mRNA,可用以检测外源因素对降钙素受体mRNA表达的影响。
英文摘要
Objective To investigate the expression of calcitonin receptor mRNA in the osteoclasts of the resorbing deciduous teeth.Methods After fixing the collected deciduous teeth, toluidine blue was performed and tartrate-resistant acid phosphatase (TRAP) staining was used to identify the osteoclasts on the resorbing surface of human deciduous teeth and in situ hybridization of calcitonin receptor mRNA to show its existence.Results There were a number of TRAP positive osteoclasts on the root surface which showed the expression of calcitonin receptor mRNA.Conclusion On the resorbing surface of human deciduous teeth there are osteoclasts that express calcitonin receptormRNA, so it is feasible to use this kind of osteoclasttotestthe effect of external fac- tors on the expression of CTR mRNA.
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