Comfort义齿粘附剂的体外细胞毒性评价
In vitroCytotoxicity Evaluation of Comfort Denture Adhesive
作者：赵 克1,程祥荣2,高 燕1,韩光丽2
Author：ZHAOKe1,CHENGXiang-rong2,GAO Yan1,HANGuang- li2
收稿日期：2004-04-25 年卷（期）页码：2004,22(02):162-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：义齿粘附剂,人口腔成纤维细胞,细胞毒性,四唑盐比色法,
Key words：denture adhesive,human oral fibroblast cell,cytotoxicity,tetrazolium bromide colorimetric assay,
基金项目：
中文摘要
目的 评价作者自行研制的新型Comfort义齿粘附剂(Comfort-DA)的体外细胞毒性。方法 Comfort-DA置于细胞培养液72 h制备出材料的浸提液,用同一培养液再稀释为50%、75%浓度后分别加入含人口腔成纤维细胞悬浮液的培养板中,其中空白对照组仅加入新鲜培养液。3块培养板分别继续在5% CO2、37℃培养箱内开放培养 2 d、3 d和4 d后,四唑盐比色法观察细胞的活性以研究细胞增殖率,用分光光度计以490 nm波长测光密度值(OD), 组间方差分析并评定该材料的毒性级。结果 空白对照组、Comfort-DA的50%和75%浓度组间OD值的差别有显著性意义(P
英文摘要
Objective The purpose of this study was to evaluate thein vitrocytotoxicity of novel Comfort denture adhesive (Comfort-DA), whichwas developed by the authors, to human oral fibroblasts (HOFs).Methods Asample of Comfort-DAwas prepared and extracted in culturing medium to prepare the eluate. Then the eluate was diluted by culturing medium to 50% and 75% concentration for the assessment of cytotoxicity by tetrazolium bromide (MTT) colorimetric assay. Wells containing freshme- dium alone were served as control. Cell viability was recorded by optical density after culturing in an atmosphere of 5% CO2and 95% air at 37℃for2, 3 and 4 days, respectively. The viability ofHOF cellswas evaluated byMTTassayto investigate cell pro- liferation. Optical density (OD) was measured by a spectrophotometer at 490 nm. Then evaluating the cytotoxicity grade in test groups according to the means of cell proliferation. ANOVAwas used to test the statistical significance.Results The statistical analysis of the results ofMTT cytological assay indicated significant difference (P
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