Objective To test whetherPorphyromonas gingivalis(P. gingivalis) could produce bacterial signal molecule, bis-(3’-5’)-cyclic dimeric adenosine monophosphate (c-di-AMP) and lay the foundation for explorations of its roles in life metabolism and periodontitis immunity ofP. gingivalis.MethodsP. gingivalisstandard strain ATCC33277 was used as the experimental strain to extract nucleic acids from the bacteria. Then, c-di-AMP was detected using high performance liquid chromatography coupled with mass spectrometry (HPLC-MS/MS). Subsequently, HPLC was used to validate the sample further. Results Based on the signal/noise (S/N) for 3∶1, the limit of determination of HPLC-MS/MS for peak time of c-di-AMP standard substances was 7.49 min and nucleic acid extractions fromP. gingivaliswas 8.82 min (S/N>3). Further confirmation of HPLC showed that nucleic acid extractions from bothP. gingivalisand c-di-AMP standard substances presented goal absorbent peaks at 15.7 min, with the same ultraviolet absorbent spectrum. Conclusion The nucleic acid extractions fromP. gingivaliscontained c-di-AMP, which shows thatP. gingivaliscould produce c-di-AMP.
