细菌感染影响牙周组织细胞表达环鸟苷-腺苷合成酶的实验研究

ObjectiveThis work aims to determine the effect of cytosolic bacteria on the expression of cyclic GMP-AMP synthase (cGAS) in human periodontal ligament cells (hPDLCs) and gingival tissues.MethodsThe ability ofPorphyromonas gingivalis(P. gingivalis) to invade hPDLCs was detected using laser scanning confocal microscope assay at a multiplicity of infection of 10.P. gingivalis-infected cells were sorted by fluorescence-activated cell sorting (FACS). Then, quantitative real time reverse transcription polymerase chain reaction (qRT-PCR) and Western blot were used to detect cGAS expression in infected cells. Finally, the location and expression of cGAS in inflammatory and normal gingival tissues were investigated by immunohistochemistry.ResultsP. gingivalisactively invaded hPDLCs. Moreover, cGAS expression significantly increased inP. gingivalis-infected cells. Although cGAS was expressed in the epithelial and subepithelial cells of both inflamed andnormal gingival tissues, cGAS expression significantly increased in inflamed gingival tissues.ConclusionCytosolic bacteria can upregulate cGAS expression in infected cells. These data suggest that cGAS may act as pattern-recognition receptors and participate in recognizing cytosolic nucleic acid pathogen-associated molecular patterns.