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论文摘要

复合膜异位诱导成骨研究

Heterotopic complex membrane induces bone formation

作者:林崇韬 李红艳 闫嘉晴

Author:LIN Chong-tao, LI Hong-yan, YAN Jia-qing

收稿日期:2009-08-25          年卷(期)页码:2009,27(04):357-

期刊名称:华西口腔医学杂志

Journal Name:West China Journal of Stomatology

关键字:胶原,聚乳酸/聚羟基乙酸共聚体,重组人骨形态发生蛋白-2,

Key words:collagen,polylactic-co-glycolic acid,recombinant human bone morphogenetic protein-2,

基金项目:

吉林省科技厅自然科学基金资助项目(20020658)

中文摘要

目的将基因重组人骨形态发生蛋白-2(rhBMP-2)与胶原、聚乳酸/聚羟基乙酸共聚体(PLGA)复合,制成rhBMP-2/Co/PLGA复合膜,观察其异位诱导成骨情况,评价其生物特性。方法取雄性昆明小鼠48只,随机分成Co/PLGA复合膜组与rhBMP-2/Co/PLGA复合膜组。在小鼠右后肢大腿肌间隙内分别植入2种复合膜材料。术后7、14、28 d,各组分别处死8只动物,标本行肉眼、软X线照相和组织学观察。结果2组动物术后情况良好。Co/PLGA复合膜组术后7 d复合膜开始溶解,14 d膜结构不连续,28 d大部分溶解;软X线和组织学观察未见异位骨生成。rhBMP-2/Co/PLGA复合膜组软X线和组织学观察均有骨样结构形成,与Co/PLGA复合膜组相比复合膜降解速度缓慢。结论Co/PLGA是rhBMP-2的良好载体,参与rhBMP-2诱导成骨活性的表达,使其在局部发挥更持久的生物活性。

英文摘要

Objective The composite membrane was made by recombinant human bone morphogenetic protein-2 (rhBMP-2), collagen(Co), polylactic-co-glycolic acid(PLGA). To observe the ectopic bone formation and evaluate the capability of rhBMP-2/Co/PLGA complex membrane. Methods 48 male Kunming adult mice were divided into two groups randomly: Co/PLGA complex membrane group and rhBMP -2/Co/PLGA complex membrane group. After etherized, an incision was made on the outer muscle and two groups of complex membranes were implanted into the right side hind limb muscle pouch. 8 mice from each group were sacrificed at 7, 14, 28 d. The hind limbs were removed, examined by soft X-ray, HE staining and light microscope examination. Results Wounds healed well, no infections and rejections were observed. In the Co/PLGA group, partial implanted membrane degradated and absorbed at 7 d. The membrane collagen fiber appeared loose at 14 d. The membrane lost its intact to disrupted at 28 d. No ectopic bone formation was found. In the rhBMP-2/Co/PLGA group, ectopic bone formation was found by eye, soft Xray and histological examinations. The rhBMP-2/Co/PLGA complex membrane degraded slower than Co/PLGA complex membrane. Conclusion Co/PLGA can provide a carrier for rhBMP-2 to have stronger effect of biological activity.

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