基质金属蛋白酶-8在人和大鼠牙胚发育钟状期的表达
Expression of Matrixmetalloproteinase-8 on the Bell-stage in Human and Rat Tooth Development
作者：郝玉庆,牛忠英,王国荃,周学东,胡涛
Author：HAO Yu-qing1,NIU Zhong-ying2,WANG Gou-quan3,ZHOUXue-dong1,HUTao1
收稿日期：2004-02-25 年卷（期）页码：2004,22(01):26-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：基质金属蛋白酶-8,牙胚发育,牙本质形成,
Key words：matrixmetalloproteinase-8,tooth development,dentin formation,
基金项目：博士点基金资助项目(20030610052)
中文摘要
目的 研究基质金属蛋白酶-8(MMP-8)在牙胚发育牙本质形成过程中的表达。方法 免疫组织化学方法检测MMP-8在人牙胚发育钟状期的表达;原位杂交方法检测MMP-8 mRNA在大鼠牙胚发育钟状期的表达。结果 MMP-8在人牙胚钟状晚期硬组织形成期成牙本质细胞和牙本质基质阳性表达,近髓腔处,牙本质染色更深;MMP-8 mRNA在大鼠牙胚发育钟状早期个别分化的成牙本质细胞表达阳性,钟状晚期,即硬组织形成期,MMP-8mRNA在成牙本质细胞表达强阳性。结论 MMP-8可能参与了人和大鼠牙胚发育过程牙本质基质改建。
英文摘要
Objective To study the expression of MMP-8 in human and rat tooth development.Methods Immunohis- tochemistry was used to detect the localization of MMP-8 protein while in situ hybridization was used to examine the expression of MMP-8 mRNA.Results The expression ofMMP-8 proteinwas localized in odontoblast and dentin matrix at the later bell stage in human tooth germ. The dentin was denser close to the pulp cavity. The expression ofMMP-8 mRNAwas found in very few polar- ized odontoblast at the early bell stage and all polarized odontoblast at the later bell stage in rat tooth germ.Conclusion The re- sults suggested thatMMP-8 involved in dentinmatrix rebuilding in the process of dentin formation in human and rat dental develop- ment.
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